• USA Home
  • P0110 - Anti-Prion Protein antibody, Mouse monoclonal

P0110 Sigma-Aldrich

Anti-Prion Protein antibody, Mouse monoclonal

clone 8H4, purified from hybridoma cell culture

Synonym: Anti-AA960666, Anti-CD230, Anti-PRIP, Anti-PrP<C>, Anti-PrP, Anti-PrPSc, Anti-Prn-i, Anti-RP23-401J24.1, Anti-Sinc, Monoclonal Anti-Prion Protein antibody produced in mouse



Related Categories ALS, Prion Disease, and others, Alphabetical Index, Antibodies, Antibodies for Neurobiology, Antibodies for Neurodegenerative Diseases Research,
conjugate   unconjugated
clone   8H4, monoclonal
biological source   mouse
application(s)   electron microscopy: suitable
  flow cytometry: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  indirect ELISA: suitable
  western blot: 2-4 μg/mL using mouse brain extract
species reactivity   monkey, bovine, rat, human, sheep, mouse
mol wt   antigen 25-35 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2b
antibody product type   primary antibodies
concentration   ~1.5 mg/mL
UniProt accession no.   P04156
Gene Information   bovine ... PRNP(281427)
human ... PRNP(5621)
mouse ... Prnp(19122)
rat ... Prnp(24686)
sheep ... PRNP(493887)


General description

Anti-Prion Protein antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma 8H4 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from Prnp-/- mice immunized with recombinant murine PrPc. Prion protein (PrP) is mapped to human chromosome 20p13. PrP is constitutively expressed in brain and other tissues of healthy humans and animals and is present in high levels at the synapse.


The antibody epitope resides within amino acids 145-180 of human prion.


Recombinant MoPrp residues 121-231

recombinant mouse PrPC.


Anti-Prion Protein antibody, mouse monoclonal may be used in:
• immunoblotting
• flow cytometry
• immunocytochemistry
• immunoprecipitation
• immunoelectron microscopy
• immunohistochemistry
• enzyme linked immunosorbent assay

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Prion-related diseases are fatal neurodegenerative disorders also known as transmissible spongiform encephalopathies (TSEs). Prion plaques are of three types: unicentric (single, compact core), multicentric (two or more cores and definite border), and diffuse plaques without a definite central core. Disease-associated prion protein specifically inhibits the proteolytic β-subunits of the 26S proteasome. This may clarify the mechanism of cell death by the prion protein. Prion protein may be involved in copper utilization, serving to buffer copper at the synaptic cleft or to mediate re-uptake of copper into the presynapse. Alternatively, bound copper may influence PrP binding characteristics. The PrP-copper complex may be crucial for synaptic homeostasis as a result of its antioxidant activity.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Certificate of Origin

Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

P6488 Monoclonal Anti-Prion Protein antibody produced in mouse, clone F89/160.1.5, purified immunoglobulin, buffered aqueous solution
P5999 Monoclonal Anti-Prion Protein antibody produced in mouse, 1 mg/mL, clone IPC1, purified immunoglobulin, buffered aqueous solution

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?