P6001 Sigma-Aldrich


Synonym: 1,4-Benzenediamine, 1,4-Diaminobenzene, 1,4-Phenylenediamine

  • CAS Number 106-50-3

  • Linear Formula C6H4(NH2)2

  • Molecular Weight 108.14

  •  Beilstein Registry Number 742029

  •  EC Number 203-404-7

  •  MDL number MFCD00007901

  •  PubChem Substance ID 24898724



vapor density   3.7 (vs air)
vapor pressure   1.08 mmHg ( 100 °C)
description   anti-fade reagent
assay   98% (HPLC)
bp   267 °C(lit.)
mp   138-143 °C (lit.)



Anti-fade reagent added to the mounting medium in fluorescence microscopy to retard photobleaching of fluorescein and other fluorescent dyes.

Biochem/physiol Actions

Electron Donor. Reduces cytochrome  c.


Darkens in storage.


1 kg in glass bottle

50, 100, 250 g in glass bottle

Safety & Documentation

Safety Information

Signal word 
Target organs 
Heart, Kidney, Musculo-skeletal system
UN 1673 6.1 / PGIII
WGK Germany 
Flash Point(F) 
230 °F
Flash Point(C) 
110 °C

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the solubility of p-Phenylediamine, Product P6001?
This product is soluble in ethanol at a concentration of 50 mg/mL, with heating. A clear red to brown solution is observed.
For what applications is p-Phenylediamine, Product P6001, used?
This is an antifade reagent that is used as a fluorescent stabilizer. Do not confuse the p-phenylenediamine with o-phenylenediamine, which is used as a peroxidase substrate in ELISA procedures.
What is the working concentration for p-Phenylediamine, Product P6001, when used as an antifade agent?
J. Immunological Methods, 43, 349 (1981) describes a solution that will reduce the fading of fluorescent materials. The recipe for this solution is: "Add 10 mL of phosphate-buffered saline (0.01 M PO4, pH 7.4 in 0.15 M NaCl) containing 100 mg of p-phenylenediamine to 90 ml of glycerol. The final pH should be adjusted to approximately 8.0 with 0.5 M carbonate-bicarbonate buffer (pH 9.0)."
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Protocols & Articles


US EPA Method 8270 (Appendix IX): GC Analysis of Semivolatiles on SLB®-5ms (30 m x 0.25 mm I.D., 0.50 μm)

From our library of Articles, Sigma-Aldrich presents US EPA Method 8270 (Appendix IX): GC Analysis of Semivolatiles on SLB®-5ms (30 m x 0.25 mm I.D., 0.50 μm)
Keywords: Chromatography, Gas chromatography, Purification

Peer-Reviewed Papers


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