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P6757 Sigma-Aldrich

PIPES

≥99% (titration)

Synonym: 1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)

  • CAS Number 5625-37-6

  • Empirical Formula (Hill Notation) C8H18N2O6S2

  • Molecular Weight 302.37

  •  Beilstein/REAXYS Number 817713

  •  EC Number 227-057-6

  •  MDL number MFCD00006159

  •  PubChem Substance ID 24898793

  •  NACRES NA.25

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffers A to Z, Good’s Buffers
Quality Level   300
  400
assay   ≥99% (titration)
form   crystalline powder
useful pH range   6.1 - 7.5
pKa (25 °C)   6.8
mp   >300 °C (lit.)
solubility   1 M NaOH: 20 + 80 mL g, clear, colorless
Featured Industry   Diagnostic Assay Manufacturing
SMILES string   OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O
InChI   1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)
InChI key   IHPYMWDTONKSCO-UHFFFAOYSA-N

Description

General description

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Application

Glutaraldehyde fixation of plant and animal tissue samples can cause loss of lipid, leading to apparent morphological changes. Lipid loss and artifacts were minimized when PIPES was used to buffer the glutaraldehyde fixative.,
Alkaline phosphatase activity is lost selectively from certain rat hepatocyte organelles when fixed for ultracytochemistry with cacodylate-buffered glutaraldehyde. When PIPES was used as buffer, retention of activity was 60% greater.
Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer.

Packaging

1 kg in poly bottle

25 kg in poly drum

25, 100, 500 g in poly bottle

Linkage

Sigma-Aldrich offers BioPerformance Certified cell culture-tested PIPES (Product No. P1851) as well as several different salts for convenience in buffer preparation, and PIPES BioXtra (Product No. P8203) which is tested for the presence of trace metals.

Preparation Note

Buffers can be prepared by adding a solution of base to PIPES free acid to titrate to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt to titrate to the appropriate pH.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Peer-Reviewed Papers
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References

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