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PROTRA Millipore

ProteoPrep® Reduction and Alkylation Kit

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Description

Application

The ProteoPrep Reduction & Alkylation Kit contains the necessary reagents to conveniently reduce and alkylate disulfide binds in preparation for 2D gel electrophoresis. These reagents are compatible with chaotropic extraction reagents and are conveneintly packaged to save time and increase efficiency. The kit contains five vials of both the reducing agent, tributylphosphine (TBP), and the alkylating agent, iodoacetamide.

Features and Benefits

• Tributylphosphine is supplied safely as a 200 mM solution in N-methyl-2-pyrrolidine
• Reduction and alkylation of protein samples increase 2D spot resolution
• Conveniently packaged components save time and increase efficiency
• Compatibility with chaotropic extraction reagents simplifies sample preparation

Legal Information

ProteoPrep is a registered trademark of Sigma-Aldrich Co. LLC

Protocols & Articles

Articles

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA™

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as ...
LSI Issue 24
Keywords: Alkylations, Amino acid analysis, Cell culture, Cell disruption, Centrifugation, Digestions, Gene expression, High performance liquid chromatography, Immobilization, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Phase transitions, Precipitation, Proteomics, Reductions, Transfection, Western blot

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