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R1755 Sigma-Aldrich

Rhodamine B isothiocyanate

BioReagent, mixed isomers, suitable for protein labeling

Synonym: RBITC

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, Biochemicals and Reagents, Derivatization Reagents, Derivatization Reagents HPLC,
product line   BioReagent
labeling efficiency with bovine albumin   >70%
fluorescence   λex 543 nm; λem 580 nm in methanol
suitability   suitable for protein labeling
storage temp.   −20°C
SMILES string   [Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4C(O)=O)N=C=S)=[N+](\CC)CC)c1
InChI   1S/C29H29N3O3S.ClH/c1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)22-12-9-19(30-18-36)15-25(22)29(33)34;/h9-17H,5-8H2,1-4H3;1H
InChI key   YVSWPCCVTYEEHG-UHFFFAOYSA-N

Description

General description

Rhodamine B isothiocyanate (RhBITC) is an organic fluorescence dye and is widely used as a fluorescent tag in a variety of biological applications. RhBITC is adsorbed onto Ag on silica or polystyrene beads. It exhibits not only a strong surface-enhanced Raman scattering (SERS) signal but also a measurable amount of fluorescence. The RhBITC-modified Ag-deposited silica or polystyrene beads disperse well in ethanol. They are also readily coated in water with polyelectrolytes for their further derivatization with biological molecules of interest that can bind to target molecules.

Application

Rhodamine B isothiocyanate is used for the following applications:
• Rhodamine isothiocyanate labeling
• Rhodamine-labeled synthetic APF
• Preparations of protein and fluorescent conjugates
• Fluorescent red dye

Packaging

1 g in glass bottle

100, 500 mg in glass insert

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Precautionary statements 
Personal Protective Equipment 
RIDADR 
UN 3335
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Sensor Dyes

In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular syst...
Monika Baeumle
BioFiles 2011, 6.3, 23.
Keywords: Applications, Biochemistry, Biological processes, Cancer, Cardiovascular, Central Nervous System, Diseases, Extinction coefficient, Indicators, Inflammation, Methods, Microscopy, Neurotransmission, Sol-gel, Type

Peer-Reviewed Papers
15

References

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