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R3131 Sigma-Aldrich

Bln I from Brevibacterium linens

buffered aqueous glycerol solution



Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
grade   for molecular biology
form   buffered aqueous glycerol solution
concentration   10,000 units/mL
shipped in   wet ice
storage temp.   −20°C



Enzyme in storage buffer stable at −15 to −25 °C. Store at ­-20 °C.

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SH (B3657).


Recognition sequence: 5′-C/CTAGG-3′
Ligation and recutting results: After 2-10-fold Bln I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >70% of fragments can be ligated, and >90% recut.
Heat inactivation:. This enzyme is not heat inactivated at 65 °C for 15 minutes.

Physical form

Solution in 10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 100 mM KCl, 10 mM 2-mercaptoethanol, 100 μg/ml BSA, 50% glycerol (v/v) at 4°C


BlnI is a restriction endonuclease that is used in molecular biological methods to cleave DNA at the recognition site 5′-C/CTAGG-3′, generating fragments with 5′-cohesive ends.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Restriction Enzyme Buffer Reference

Our restriction enzyme collection has been optimized for digestion using five unique buffers. Reference below the enzyme activity in each buffer (table 1), and the preferred buffer for each enzyme (t...
Keywords: Buffers, Digestions, Enzyme activity, PAGE, Solid phase extractions

Restriction Enzyme Double Digest Buffer Guide

Our restriction enzyme collection has been optimized for digestion using five unique buffers. When using two restriction enzymes at once, first check the enzyme activities in each buffer, using table...
Keywords: Buffers, Digestions, Solid phase extractions


Restriction Enzyme Digest Protocol

Our restriction enzyme collection has been optimized for digestion using five unique buffers. When digesting DNA using a single enzyme, use the buffer supplied with the enzyme (also identified on tab...
Keywords: Buffers, Digestions, Purification

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers


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