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R9507 Sigma-Aldrich

Taq I from Thermus aquaticus

recombinant, expressed in E. coli (Strain that carries a Taq I overproducing plasmid.), Restriction Enzyme



Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
recombinant   expressed in E. coli (Strain that carries a Taq I overproducing plasmid.)
grade   for molecular biology
form   buffered aqueous glycerol solution
concentration   10,000 units/mL
shipped in   wet ice
storage temp.   −20°C


Other Notes

Supplied with 10x Restriction Enzyme Buffer SB (B8781).

Comment: Taq I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam+ strains). Taq I is inefficient in digesting single stranded DNA . Overlapping dam methylation will block cleavage by Taq I. Activity of the enzyme is enhanced by BSA and higher temperatures, >37 °C. Optimal temperature is 65 °C.


Recognition sequence: 5′-T/CGA-3′
Ligation and recutting results: After 2-10-fold Taq I overdigestion of 1 μg λ DNA substrate, results in >95% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl, 300 mM KCl, 7 mM 2-mercaptoethanol, 50% glycerol (v/v) at 4 °C


TaqI is a restriction endonuclease used in molecular biology applications to cleave DNA moledcules at the recognition site 5′-T/CGA-3′, generating fragments with 5′-cohesive ends.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Restriction Enzyme Buffer Reference

Our restriction enzyme collection has been optimized for digestion using five unique buffers. Reference below the enzyme activity in each buffer (table 1), and the preferred buffer for each enzyme (t...
Keywords: Buffers, Digestions, Enzyme activity, PAGE, Solid phase extractions


Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson. The ability of certain E. coli strains to ...
Keywords: Asymmetric synthesis, Buffers, Cloning, Degradations, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Indirect Immunofluorescense, Methylations, Molecular biology, Molecular biology techniques, Peptide synthesis, Polymorphisms, Reproduction, Separation, Sequencing

Restriction Enzyme Digest Protocol

Our restriction enzyme collection has been optimized for digestion using five unique buffers. When digesting DNA using a single enzyme, use the buffer supplied with the enzyme (also identified on tab...
Keywords: Buffers, Digestions, Purification

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers


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