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R9532 Sigma-Aldrich

Pyocyanin, Ready Made Solution from Pseudomonas aeruginosa

5 mg/mL in DMSO, 0.2 μm filtered

Synonym: 5-Methyl-1(5H)-phenazinone, Pyocyanine, Sanasin, Sanazin

  • CAS Number 85-66-5

  • Empirical Formula (Hill Notation) C13H10N2O

  • Molecular Weight 210.23

  •  MDL number MFCD01794662

  •  PubChem Substance ID 329824047

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Properties

Related Categories Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules, Cell Biology, Cell Signaling and Neuroscience, Cell Stress,
InChI Key   YNCMLFHHXWETLD-UHFFFAOYSA-N
sterility   0.2 μm filtered
assay   ≥98% (HPLC)
form   DMSO solution
concentration   5 mg/mL in DMSO
shipped in   dry ice
storage temp.   −20°C

Description

Application

Pyocyanin has been used:
• To disable the ability of forkhead box A2 (FOXA2) to regulate goblet cell hyperplasia and metaplasia (GCHM) and mucin expression.
• To activate nuclear factor (erythroid-derived 2)-like 2 (NRF2) through the reactive oxygen species (ROS)-inducible epidermal growth factor receptor (EGFR)-phosphoinositide 3-kinase (PI3K) cellular signal transduction pathway and its downstream effectors.
• To stimulate Pseudomonas aeruginosa PAO1 cell line adhesion and invasion in human lung carcinoma A549 cells via reactive oxygen species (ROS) production.

Biochem/physiol Actions

Pyocyanin, a redox-active phenazine, is an electron receptor, which stimulates redox cycling in bacteria, liver cells, and human epithlial cell lines. It enhances oxidative metabolism, which increases the formation of intracellular reactive oxygen species (ROS) via reduction of NADPH. Pyocyanin also increases the release of the neutrophil chemoattractant IL-8 by airway epithelial cells both in vitro and in vivo. This involves signal transduction pathways that include oxidants, protein tyrosin kinases and MAP-kinases. IL-8 secretion by these cells is in synergy with inflammatory cytokines. Pyocyanin accelerates neutrophil apoptosis in vitro. Mice infected with a pyocyanin-deficient strain of P. aeruginosa showed elevated levels of neutrophils and neutrophil chemokines and cytokines, as well as compromised bacterial clearance from the lungs compared with mice infected with a wild type strain. This suggests that pyocyanin production by P. aeruginosa suppresses the acute inflammatory response by pathogen-driven acceleration of neutrophil apoptosis and by reducing local inflammation, and that this is advantageous for bacterial survival.

General description

Pyocyanin is a secondary metabolite, which is produced by Pseudomonas aeruginosa.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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