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RDD002 Sigma-Aldrich

HEPES

anhydrous, free-flowing, Redi-Dri, ≥99.5%

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein Registry Number 883043

  •  EC Number 230-907-9

  •  MDL number MFCD00006158

  •  PubChem Substance ID 329824215

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Properties

grade   Redi-Dri
  anhydrous
InChI Key   JKMHFZQWWAIEOD-UHFFFAOYSA-N
assay   ≥99.5%
quality   free-flowing
pH   5.0-6.5 (25 °C, 238 g/L)
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5

Description

Packaging

1 kg in poly bottle

2.5 kg in poly drum

Application

HEPES has been used:
• In the novel vesicle-budding protocol as a component in stock solution and buffer solutions
• As a component in intracellular solution for the preparation of brain-slice recording
• As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification
• To maintain the pH at 7 in thermal denaturation assay of purified protein
• In isocratic elution of protein using gel filtration
• Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Enjoy the Free-flowing Experience - Redi-Dri™

What is the most frustrating moment you have experienced in the lab? Inadequate chemicals for the reaction? Or rock-solid material as you open your bottle when you only need a small amount? If you ha...
Keywords: Environmental

Protocols

Redi-Dri™ FAQs

A time study was performed to test the Red-Dri Packaging System against standard packaging. Equivalent units of both packaging systems containing potassium chloride (KCl) were introduced to a control...
Keywords: Absorption

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Redi-Dri™ – The world of free-flowing hygroscopic products

The Redi-Dri™ packaging system eliminates the common issue of clumping that is noted with many hygroscopic salts and buffers and provides free-flowing, ready-to- use material at all times. Don’t wast...
Keywords: Buffers, Chromatin immunoprecipitation, Environmental, Reductions, Sample preparations

Redi-Dri™ – 뭉치고 덩어리지지 않는 시약의 세계

Redi-Dri™ 패키징 시스템은 기존에 흡습성이 강한 시약에서 흔하게 볼 수 있는 뭉침과 덩어리짐을 방지하여 균일한 유동성으로 시약을 바로 시험에 이용이 가능하도록 디자인 된 제품입니다. 더 이상 시작 전부터 굳어버리는 제품으로 소중한 시간을 낭비하지 마세요. Redi-Dri™를 사용해보세요!

Peer-Reviewed Papers
15

References

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