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SAB1305536 Sigma

MONOCLONAL ANTI-HA TAG antibody produced in mouse

clone 12CA5, IgG fraction of antiserum, buffered aqueous solution

Synonym: Tag from influenza hemagglutinin protein

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Properties

Related Categories Alphabetical Index, Antibodies, H1-HE, Primary Antibodies
biological source   mouse
antibody form   IgG fraction of antiserum
clone   12CA5, monoclonal
form   buffered aqueous solution
application(s)   western blot: 1:1000
isotype   IgG2b
UniProt accession no.  
shipped in   wet ice
storage temp.   −20°C

Description

Physical form

Supplied in PBS with 0.09% (W/V) sodium azide

Application

MONOCLONAL ANTI-HA TAG antibody produced in mouse has been used in Western blotting.

Biochem/physiol Actions

Influenza virus hemagglutinin (HA) acts as a key immunogen in avian influenza virus (AIV) vaccines. Activated HA facilitates virus attachment to oligosaccharide receptors on host cell surface and is involved in penetration through fusion following conformational modifications in the endolysosomal compartment. 226 and 228 residues in HA of the H3 subtype play a key role in host range restriction and receptor specificity.

General description

Influenza virus hemagglutinin (HA) precursor is encoded by the gene mapped on segment 4. The encoded protein exists as trimers on the envelope of the virus and is a 76kDa surface glycoprotein.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Antibody Explorer
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Search & filter by validated application (ie, WB, IF/ICC, IHC, ELISA, etc,) host species, clonality (monoclonal, polyclonal and recombinant) and more. Learn from technical information, product highli...
Keywords: Amplification, Buffers, Enzyme-linked immunosorbent assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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