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SAB2702197 Sigma-Aldrich

Monoclonal Anti-GFP antibody produced in mouse

clone GT859, affinity isolated antibody

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Properties

Related Categories Alphabetical Index, Antibodies, GD-GL, Primary Antibodies
conjugate   unconjugated
clone   GT859, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunofluorescence: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: 1000-10000
species reactivity   mouse
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
isotype   IgG2b
antibody product type   primary antibodies
concentration   1mg/mL

Description

General description

Green fluorescent protein (GFP) is a fluorescent protein. It is mainly isolated from the luminous organ of the jellyfish Aequorea victoria.

Immunogen

Full length GFP recombinant protein

Application

Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, ICC/IF: 1:100-1:2000, IHC-Fr: Assay-dependent dilution, IHC-P: Assay-dependent dilution, IP: 1:100-1:500, WB: 1:1000-1:10000, IHC-Wm: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Monoclonal Anti-GFP antibody has been used in gait analysis.

Physical form

Phosphate-buffered saline, no preservative added.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Green fluorescent protein (GFP) is used as a marker for gene expression. It is also used as a tag to study about protein localization in several organisms. GFP can form the intrinsic chromophore without the help of cofactors or enzymatic components.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Other Notes

Purification: Affinity purified by Protein G

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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