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  • SAB2702219 - Monoclonal Anti-6X His tag antibody produced in mouse

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SAB2702219 Sigma-Aldrich

Monoclonal Anti-6X His tag antibody produced in mouse

clone GT359, affinity isolated antibody

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Properties

Related Categories Alphabetical Index, Antibodies, HF-HI, Primary Antibodies
conjugate   peroxidase conjugate
clone   GT359, monoclonal
biological source   mouse
application(s)   western blot: 1:1000-1:20000
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
isotype   IgG3
antibody product type   primary antibodies
concentration   0.4mg/mL

Description

General description

6X His is a histidine tag that consists of a series of histidine residues at the amino or carboxy terminus of a protein.

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag

Application

Suggested starting dilutions are as follows: WB: 1:1000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Physical form

0.05M Tris, 0.15M NaCl (pH7.4). 0.025% ProClin 300 was added as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

6X His tag does not affect the structure and function of the protein and can be used in antibody production.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Other Notes

Purification: Affinity purified by Protein G

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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