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SAB4200644 Sigma-Aldrich

Monoclonal Anti-C10orf54 antibody produced in mouse

clone ORF.4, purified from hybridoma cell culture

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Properties

Related Categories Alphabetical Index, Antibodies, C1-C19, Primary Antibodies
clone   ORF.4, monoclonal
biological source   mouse
application(s)   flow cytometry: 10 μg/test using human platelets
  immunohistochemistry: suitable
species reactivity   human
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
Gene Information   human ... C10orf54(64115)

Description

General description

Monoclonal Anti-C10orf54 (mouse IgG1 isotype) is derived from the hybridoma OR.4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide.C10orf54 (Chromosome 10 open reading frame 54) is also referred to as GI24, B7 member B7-H5, VISTA (V-domain Ig suppressor of T cell activation) and Dies1. It is a novel surface glycoprotein belonging to the B7 immunoglobulin superfamily. It consists of a single IgV domain with 3 additional cysteine residues and a cytoplasmic tail. It is expressed on mature myeloid APCs (antigen-presenting cells) including peritoneal macrophages, mature BMDC (bone marrow-derived dendritic cell), neutrophils and CD11c+ DCs, and to a lesser quantity in T cells and activated Treg cells.

Immunogen

synthetic peptide corresponding to a sequence at the internal region of human C10orf54

Application

Phosphotyrosine antibody inhibitor has been used in flow-cytometry and immunohistochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Studies suggest that C10orf54 (Chromosome 10 open reading frame 54) functions as a co-inhibitory ligand and restricts the mechanism of T cell proliferation and cytokine production. It was also suggested that C10orf 54 acts as a positive regulator as well as a substrate of MT1-MMP (trans-membrane type 1-matrix metalloproteinase) during tumor growth of collagen matrix, which regulates invasive growth.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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