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SAB4200655 Sigma-Aldrich

Monoclonal Anti-NCAPH2 antibody produced in rat

clone 5F2G4, purified from hybridoma cell culture

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, N-ND, Primary Antibodies
clone   5F2G4, monoclonal
biological source   rat
application(s)   flow cytometry: 1-2 μg/test using using HeLa cells
  immunoblotting: 2-4 μg/mL using using whole extracts of HeLa cells
  immunofluorescence: 0.25-0.5 μg/mL using using HeLa cells
species reactivity   human, rat, mouse
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   Q6IBW4
Gene Information   human ... NCAPH2(29781)
mouse ... Ncaph2(52683)
rat ... Ncaph2(300149)

Description

General description

Non-SMC condensin II complex subunit H2 (NCAPH2) is one of the subunits of the condensin II complex. The gene encoding it is localized on human chromosome 22q13.

Immunogen

a human NCAPH2 (full-length) recombinant fusion protein

Application

Monoclonal Anti-NCAPH2 antibody produced in rat has been used in immunostaining.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Non-SMC condensin II complex subunit H2 (NCAPH2) binds to mitotic chromosomes and is associated with mitosis. Mutations in the gene encoding NCAPH2 have been shown to impair T-cell functions in mice. The NCAPH2 gene is associated with mitosis and meiosis, which predicts that its methylation influences apoptosis of neural cells. NCAPH2 methylation causes loss of hippocampal function and cognitive dysfunction leading to progressive dementia.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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