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  • SAB4200670 - Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse

SAB4200670 Sigma-Aldrich

Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse

clone NOQ7.5.4D, purified from hybridoma cell culture


  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, MUE-MZ, Primary Antibodies
clone   NOQ7.5.4D, monoclonal
biological source   mouse
application(s)   immunoblotting: 1.25-2.5 μg/mL using Rat skeletal muscle lysate.
  immunohistochemistry: 2.5-5 μg/mL using pronase digested, formalin-fixed, paraffin-embedded rabbit tongue/leg sections and Biotin/ExtrAvidin®-Peroxidase staining system.
species reactivity   canine, mouse, human, pig, rabbit, chicken, guinea pig, goat, hamster, rat, sheep, feline, bovine
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL


General description

Monoclonal Anti-Myosin (Skeletal, Slow) (mouse IgG1 isotype) is derived from the NOQ7.5.4D hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Myosin is a hexameric protein containing two heavy chain subunits and four light chain subunits. It is a major protein component of heart and skeletal muscles. Muscle myosin heavy chain (MHC) different isoforms are expressed depending on the physiological function of the muscle: skeletal, cardiac, smooth and non-muscle.


Myosin, purified from myofibrils isolated from human skeletal muscle,


Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse has been used in immunobloting, immunohistology, Solid-phase radioimmunoassay (RIA), enzyme linked immunosorbent assay (ELISA) and immunoelectronmicroscopy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Biochem/physiol Actions

Myosin are actin-dependent motors. Mutations in myosins genes are associated with myopathies, blindness and hearing loss. They are involved in muscle contraction, vision, hearing, cell movements and cell invasion.

Myosin is the element responsible for energy transduction and force development in heart and skeletal muscles. Myosin is known to interact with actin in muscle and in nonmuscle cells. Slow myosin, also known as β-myosin heavy chain (β-MHC) is expressed during embryogenesis as part of the cardiac myogenic program under the control of NK-2 homeobox protein-5 (NKX-2.5), myocyte enhancer factor-2C (MEF-2C), and GATA-4/5/6.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles


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