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  • SAB4200678 - Monoclonal Anti-Collagen Type I antibody produced in mouse

SAB4200678 Sigma-Aldrich

Monoclonal Anti-Collagen Type I antibody produced in mouse

clone COL-1, purified from hybridoma cell culture

Synonym: OI4, alpha 1, alpha 2, collagen, type I

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, CO-CP, Primary Antibodies
clone   COL-1, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  dot blot: suitable
  immunoblotting: 1-2 μg/mL using recombinant human collagen, expressed in Nicotiana tabacum
  immunohistochemistry: 3.5-7 μg/mL using frozen sections of human tonsil, human tongue or pig tongue
species reactivity   rat, deer, human, pig, bovine
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL


General description

Collagen type Iα1 (COL1A1) is encoded by the gene that is located on human chromosome 17q21.33. It is the most abundant extracellular matrix (ECM) protein in humans. Type 1 collagen is the major structural protein of bone, tendon, skin and cornea. The encoded protein is a heterotrimer consisting of two α1-chains and one α2-chain.

Collagens are present in the extracellular matrix and contains a chains which can differ in length.


bovine skin collagen type I


Monoclonal Anti-Collagen Type I antibody produced in mouse has been used in immunohistochemical analysis and in vitro invasion assays.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Collagen, type I (COL1A1) is an important component of the connective tissue matrix. It plays an important role in the growth and maintenance of organ and tissue integrity. This protein also participates in the process of tissue repair. COL1A1 is implicated in fibrosis.

Collagens are involved in structural roles. Additionally, they associate with various receptors and control cell proliferation, migration as well as differentiation.

Safety & Documentation

Safety Information

NONH for all modes of transport
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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