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  • SAB4200689 - Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal

SAB4200689 Sigma-Aldrich

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal

clone 5C5, hybridoma cell culture supernatant

Synonym: Actin alpha sarcomeric/skeletal, a-SCA, alpha-SCA, alpha-sarcomeric actin

  •  NACRES NA.41



Related Categories AC-AC, Alphabetical Index, Antibodies, Primary Antibodies
clone   5C5, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunoblotting: 1:500-1:1000 using rat heart tissue extracts
  immunofluorescence: 1:500-1000 using human HeLa cells.
  immunohistochemistry: 1:500 using formalin-fixed, paraffin-embedded human tongue sections and Biotin/ExtrAvidin®-Peroxidase staining system.
species reactivity   planaria(flatworm), rat, guinea pig, mouse, rabbit, chicken, bovine, Xenopus, human, fish
mol wt   antigen ~42 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   purified immunoglobulin
isotype   IgM
Quality Level   200
antibody product type   primary antibodies


General description

Monoclonal Anti-Actin (-Sarcomeric) (mouse IgM isotype) is derived from the hybridoma 5C5 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with purified rabbit striated muscle. Actin is the major cytoskeletal protein in eukaryotic cells.


purified rabbit striated muscle


Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal has been used in:
• indirect immunofluorescence staining
• immunohistochemistry
• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunofluorescence

Physical form

The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Actin plays essential roles in a number of cellular processes including cell migration, cytokinesis, vesicle transport and contractile force generation.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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