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  • SAB4200689 - Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal

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SAB4200689 Sigma-Aldrich

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal

clone 5C5, hybridoma cell culture supernatant

Synonym: Actin alpha sarcomeric/skeletal, a-SCA, alpha-SCA, alpha-sarcomeric actin

  •  NACRES NA.41

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Primary Antibodies
clone   5C5, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunoblotting: 1:500-1:1000 using rat heart tissue extracts
  immunofluorescence: 1:500-1000 using human HeLa cells.
  immunohistochemistry: 1:500 using formalin-fixed, paraffin-embedded human tongue sections and Biotin/ExtrAvidin®-Peroxidase staining system.
species reactivity   planaria(flatworm), rat, guinea pig, mouse, rabbit, chicken, bovine, Xenopus, human, fish
mol wt   antigen ~42 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   purified immunoglobulin
isotype   IgM
Quality Level   200
antibody product type   primary antibodies

Description

General description

Monoclonal Anti-Actin (-Sarcomeric) (mouse IgM isotype) is derived from the hybridoma 5C5 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with purified rabbit striated muscle. Actin is the major cytoskeletal protein in eukaryotic cells.

Immunogen

purified rabbit striated muscle

Application

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal has been used in:
• indirect immunofluorescence staining
• immunohistochemistry
• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunofluorescence

Physical form

The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Actin plays essential roles in a number of cellular processes including cell migration, cytokinesis, vesicle transport and contractile force generation.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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