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  • SAB4200711 - Anti-Human Serum Albumin antibody, Mouse monoclonal

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SAB4200711 Sigma

Anti-Human Serum Albumin antibody, Mouse monoclonal

clone HSA-11, purified from hybridoma cell culture

Synonym: ALB

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Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Primary Antibodies
species reactivity   gibbon, baboon, monkey
application(s)   immunoblotting: 2.5-5 ng/mL using human serum
  immunohistochemistry: suitable
  indirect ELISA: 0.2-0.4 μg/mL using 10 μg/ml Human Serum Albumin for coating
clone   HSA-11, monoclonal
concentration   ~1.0 mg/mL
form   buffered aqueous solution
isotype   IgG2a
mol wt   mol wt ~70 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ALB(213)
biological source   mouse

Description

Immunogen

Human Serum Albumin

General description

Anti-human serum albumin antibody, mouse monoclonal (mouse IgG2a isotype) is derived from the HSA-11 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with human serum albumin (HSA). Monoclonal anti-human serum albumin specifically recognizes human albumin derived from sera, plasma or liver cells.
HSA is encoded by the gene mapped to human chromosome 4q13.3. It is characterized with three homologous domains that assemble to form a heart-shaped molecule. HSA constitutes around 60-65% of total serum protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Other Notes

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

Biochem/physiol Actions

Human serum albumin (HAS) serves as a transport and depot protein for several endogenous ligands including fatty acids, hormones, steroids, bilirubin and heme. High intravascular concentration of HSA enables it to play an essential role in exerting colloid osmotic pressure and thereby to regulate the oncotic pressure and volume of blood. It also acts as an antioxidant and is required for exerting Donnan effect in the capillaries. Mutations in the gene is associated with the development of bisalbuminaemia or alloalbuminaemia. Deficiency of the gene leads to analbuminaemia.

Safety & Documentation

Documents

Certificate of Analysis

Safety Information

RIDADR  NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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