• USA Home
  • SAB4200715 - Anti-β-tubulin antibody, Mouse monoclonal

SAB4200715 Sigma

Anti-β-tubulin antibody, Mouse monoclonal

clone 2-28-33, purified from hybridoma cell culture

Synonym: Anti-TUBB1



Related Categories Alphabetical Index, Antibodies, Primary Antibodies, TS-TZ
biological source   mouse
clone   2-28-33, monoclonal
form   buffered aqueous solution
mol wt   mol wt ~50 kDa
species reactivity   dog, bovine, mouse, rat, monkey, human, chicken
concentration   ~1.0 mg/mL
application(s)   immunoblotting: 0.5 μg/mL using mouse embryo fibroblast NIH-3T3 cells extract
  immunofluorescence: 1-2 μg/mL using human foreskin fibroblast HS68 cells
  immunohistochemistry: suitable
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C



Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Search & filter by validated application (ie, WB, IF/ICC, IHC, ELISA, etc,) host species, clonality (monoclonal, polyclonal and recombinant) and more. Learn from technical information, product highli...
Keywords: Amplification, Buffers, Enzyme-linked immunosorbent assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?

Antibody Explorer