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SAB4200715 Sigma

Anti-β-tubulin antibody, Mouse monoclonal

clone 2-28-33, purified from hybridoma cell culture

Synonym: Anti-TUBB1

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, TS-TZ
biological source   mouse
clone   2-28-33, monoclonal
form   buffered aqueous solution
mol wt   mol wt ~50 kDa
species reactivity   dog, bovine, mouse, rat, monkey, human, chicken
concentration   ~1.0 mg/mL
application(s)   immunoblotting: 0.5 μg/mL using mouse embryo fibroblast NIH-3T3 cells extract
  immunofluorescence: 1-2 μg/mL using human foreskin fibroblast HS68 cells
  immunohistochemistry: suitable
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Search & filter by validated application (ie, WB, IF/ICC, IHC, ELISA, etc,) host species, clonality (monoclonal, polyclonal and recombinant) and more. Learn from technical information, product highli...
Keywords: Amplification, Buffers, Enzyme-linked immunosorbent assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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