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  • SAB4200716 - Anti-Vimentin antibody, Mouse monoclonal

SAB4200716 Sigma-Aldrich

Anti-Vimentin antibody, Mouse monoclonal

clone VIM-13.2, purified from hybridoma cell culture

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Primary Antibodies, V
clone   VIM-13.2, monoclonal
biological source   mouse
application(s)   immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast HS68 cell line extract
  immunofluorescence: 1.25-2.5 μg/mL using human foreskin fibroblast HS68 cells
  immunohistochemistry: suitable
species reactivity   rat, chicken, monkey, human, bovine, canine, mouse
mol wt   ~58 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified from hybridoma cell culture
isotype   IgM
antibody product type   primary antibodies
concentration   ~1.0 mg/mL


General description

Anti-Vimentin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the VIM-13.2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with human foreskin fibroblast extract. Vimentin is a type III intermediate filaments (IF) expressed in a wide range of cell types. including, pancreatic precursor cells, fibroblasts and sertoli cells. It is also expressed in trophoblastic giant cells, neuronal precursor cells, lung epithelial cells, renal tubular and stromal cells. Vimentin is also present in leucocytes, macrophages, neutrophils and mesangial cells.


human foreskin fibroblast extract


Anti-Vimentin antibody, Mouse monoclonal has been used in:
• western blotting
• indirect immunofluorescence
• immunohistochemistry

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Vimentin plays a significant role in supporting and anchoring the cellular position of organelles, such as nucleus, endoplasmic reticulum, mitochondria (either laterally or terminally). It helps to maintain the mesenchymal cells especially their shape, motility, survival during of mechanical stress. Epithelial cancers like prostate, breast, lung show an increased vimentin expression. Vimentin is also highly expressed in gastrointestinal tumors, central nervous system tumors and malignant melanoma.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


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