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SAB4200726 Sigma-Aldrich

Anti-Myosin (Smooth) antibody, Mouse monoclonal

clone hSM-V, purified from hybridoma cell culture

Synonym: Anti-SM

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Properties

Related Categories Alphabetical Index, Antibodies, MUE-MZ, Primary Antibodies
clone   hSM-V, monoclonal
biological source   mouse
application(s)   immunoblotting: suitable
  immunofluorescence: suitable
  immunohistochemistry: 10-20 μg/mL using methacarn -fixed paraffin-embedded human tonsil sections
  immunoprecipitation (IP): suitable
species reactivity   canine, quail, mouse, porcine, rat, bovine, sheep, rabbit, human
shipped in   dry ice
storage temp.   −20°C
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1.0 mg/mL

Description

General description

Anti-Myosin (Smooth) antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hSM-V hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with human uterus smooth muscle extract. Myosin together with actin belong to a family of contractile proteins. Myosin contains two heavy chains (~200 kDa each) and four light chains (15-26 kDa). The Myosin molecules which consist of two major regions: tail (rod) and head, aggregate through the tail region into filaments and interact through the head region with actin and with ATP.

Immunogen

human uterus smooth muscle extract

Application

Anti-Myosin (Smooth) antibody, Mouse monoclonal has been used in various immunochemical assays, including immunohistochemistry, immunoblotting, immunofluorescence, immunoprecipitation and fluorescence-activated cell sorting (FACS) / flow cytometry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Myosin is involved in a variety of cellular processes including secretion, cytoplasmic streaming, locomotion, phagocytosis and cytokinesis. This antibody can be a useful tool in the identification and localization of smooth muscle elements, in the diagnosis and classification of mesenchymal tumors and in myosin expression studies in normal and malignant cell proliferations.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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