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  • SAB4200726 - Anti-Myosin (Smooth) antibody, Mouse monoclonal

SAB4200726 Sigma-Aldrich

Anti-Myosin (Smooth) antibody, Mouse monoclonal

clone hSM-V, purified from hybridoma cell culture

Synonym: Anti-SM



Related Categories Alphabetical Index, Antibodies, MUE-MZ, Primary Antibodies
clone   hSM-V, monoclonal
biological source   mouse
application(s)   immunoblotting: suitable
  immunofluorescence: suitable
  immunohistochemistry: 10-20 μg/mL using methacarn -fixed paraffin-embedded human tonsil sections
  immunoprecipitation (IP): suitable
species reactivity   canine, quail, mouse, porcine, rat, bovine, sheep, rabbit, human
shipped in   dry ice
storage temp.   −20°C
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1.0 mg/mL


General description

Anti-Myosin (Smooth) antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hSM-V hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with human uterus smooth muscle extract. Myosin together with actin belong to a family of contractile proteins. Myosin contains two heavy chains (~200 kDa each) and four light chains (15-26 kDa). The Myosin molecules which consist of two major regions: tail (rod) and head, aggregate through the tail region into filaments and interact through the head region with actin and with ATP.


human uterus smooth muscle extract


Anti-Myosin (Smooth) antibody, Mouse monoclonal has been used in various immunochemical assays, including immunohistochemistry, immunoblotting, immunofluorescence, immunoprecipitation and fluorescence-activated cell sorting (FACS) / flow cytometry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Myosin is involved in a variety of cellular processes including secretion, cytoplasmic streaming, locomotion, phagocytosis and cytokinesis. This antibody can be a useful tool in the identification and localization of smooth muscle elements, in the diagnosis and classification of mesenchymal tumors and in myosin expression studies in normal and malignant cell proliferations.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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