• USA Home
  • SAB4200727 - Anti-AKT2 antibody, Mouse monoclonal

EMAIL THIS PAGE TO A FRIEND
SAB4200727 Sigma-Aldrich

Anti-AKT2 antibody, Mouse monoclonal

clone AKT2(104), purified from hybridoma cell culture

Synonym: Anti-PKB beta, Anti-Protein kinase Akt-2, Anti-RAC-beta serine/threonine-protein kinase

Purchase

Properties

Related Categories AE-AK, Alphabetical Index, Antibodies, Primary Antibodies
clone   AKT2(104), monoclonal
biological source   mouse
application(s)   immunoblotting: 10-20 μg/mL using human T lymphocyte Jurkat cell extract
species reactivity   mouse, rat, human
mol wt   ~56 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
isotype   IgG2a
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P31751
Gene Information   human ... AKT2(208)

Description

General description

Anti-AKT2 antibody, Mouse monoclonal, (mouse IgG2a isotype) is derived from the AKT2(104) hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. v-akt murine thymoma viral oncogene homolog 2 (AKT2) protein belongs to the subfamily of three closely related protein serine/threonine kinases. AKT2 is localized in the insulin-responsive tissues like adipose tissue, liver and skeletal muscle. It comprises of N-terminal regulatory pleckstrin homology (PH) domain, a central kinase domain with serine/threonine specificity and a C-terminal hydrophobic domain. It is mapped on the human chromosome at 19q13.2.

Immunogen

synthetic peptide from the internal region of human AKT2 protein, conjugated to KLH

Application

Anti-AKT2 antibody, Mouse monoclonal may be used in immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

v-akt murine thymoma viral oncogene homolog 2 (AKT2) is a key mediator of the insulin signal transduction process and is required for glucose metabolism. AKT2 plays an important role in the phosphoinositide 3-kinase (PI3K) signaling pathway and is over-expressed in a variety of malignant tumors. it is considered to be a useful molecular marker for predicting the prognosis of meningioma and breast cancer. Due to its important role in neuroblastoma tumorigenesis, it can be used as a target for developing novel therapeutics for the treatment of clinically aggressive neuroblastoma.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?