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  • SAB4200728 - Anti-Chromogranin-A antibody, Mouse monoclonal

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SAB4200728 Sigma-Aldrich

Anti-Chromogranin-A antibody, Mouse monoclonal

clone CHGA(419), purified from hybridoma cell culture

Synonym: Anti-CHGA, Anti-CgA, Anti-Pituitary secretory protein I, Anti-SP-1

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, CE-CH, Primary Antibodies
clone   CHGA(419), monoclonal
biological source   mouse
application(s)   immunoblotting: 10-20 μg/mL using human embryonic kidney 293T cell extract
  immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded Human Stomach sections
species reactivity   human
mol wt   ~50 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P10645
Gene Information   human ... CHGA(1113)

Description

General description

Anti-Chromogranin-A antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the CHGA(419) hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with synthetic peptide corresponding to the C-terminal region of human Chromogranin-A protein, conjugated to KLH. Chromogranin-A protein, also known as CgA, pituitary secretory protein I (SP-1) is the major member of the granin family of acidic secretory glycoproteins that are expressed in all endocrine and neuroendocrine cells. The protein is in neuroendocrine cells distributed throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets and the secretory vesicles of neurons.

Immunogen

synthetic peptide corresponding to the C-terminal region of human Chromogranin-A protein conjugated to KLH

Application

Anti-Chromogranin-A antibody has been used in immunohistochemistry,immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Chromogranin-A has a crucial intracellular role in secretory granule biogenesis and calcium homeostasis. Tissue-specific and context-specific proteolytic cleavage of Chromogranin-A yields polypeptides with paracrine and endocrine activity including: vasostatin, catestatin, GE25 and WE-14. Increased blood levels of Chromogranin-A have been shown in numerous inflammatory and non-inflammatory conditions, including neuroendocrine tumours (carcinoid tumors, phenochromocytomas, paragangliomas and others), renal failure, arterial hypertension, chronic heart failure and rheumatoid arthritis.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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