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SAB4200731 Sigma-Aldrich

Anti-Pan-Cadherin antibody, Mouse monoclonal

clone CH-19, purified from hybridoma cell culture

Synonym: Anti-Pan Cadherin

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Properties

Related Categories Alphabetical Index, Antibodies, P-PA, Primary Antibodies
clone   CH-19, monoclonal
biological source   mouse
application(s)   immunoblotting: 0.125-0.25 μg/mL using HeLa cell extract
  immunofluorescence: 5-10 μg/mL using HeLa cells
  immunohistochemistry: suitable
species reactivity   monkey, human, guinea pig, mouse, hamster, chicken, cat, canine, rabbit, rat, Psammomys (sand rat), frog, ferret, snake, bovine
mol wt   ~135 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P12830
  P19022
  P22223

Description

General description

N-cadherin, also known as cadherin-2 (CDH2) or neural cadherin (NCAD) is mapped to human chromosome 18q12.1. N-cadherin has ectodomains comprising of 110 amino acidsand is called the extracellular cadherin (EC) domain. EC domains are critical for the adhesion function of N-cadherin.

Immunogen

synthetic peptide corresponding to the C-terminus of chicken N-Cadherin with an extra N-terminal lysine residue coupled with KLH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

N-cadherin plays a key role in the neurulation, which involves transition of neural plate into neural tube. It is present in the synaptic membrane and dimerises upon synaptic induction and mediates synaptic activity. N-cadherin mediates cell−cell adhesion in retina but favors tumor progression in retinoblastoma. It is upregulated in carcinomas and is critical for cell invasion. Irregular distribution of N-cadherin is implicated in dilated cardiomyopathy. Mutations in the CHD2 gene may probably contribute to obsessive-compulsive disorder (OCD) and Tourette disorder (TD).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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