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  • SAB4200733 - Anti-IL6 high affinity antibody, Mouse monoclonal

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SAB4200733 Sigma-Aldrich

Anti-IL6 high affinity antibody, Mouse monoclonal

clone IL6-4G1, purified from hybridoma cell culture

Synonym: Anti-B-cell stimulatory factor 2 (BSF-2), Anti-CTL differentiation factor (CDF), Anti-Hybridoma growth factor, Anti-Interferon beta-2 (IFN-beta-2), Anti-Interleukin-6

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, IH-IM, Primary Antibodies
clone   IL6-4G1, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunoblotting: 2-4 μg/mL using concentrated supernatant of human osteosarcoma MG-63 cells treated with IL1β
  immunoprecipitation (IP): 1-2 μg/test using concentrated supernatant of human osteosarcoma MG-63 cells treated with IL1β
species reactivity   human
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P05231
Gene Information   human ... IL6(3569)

Description

General description

Anti-IL6 high-affinity antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the IL6-4G1 hybridoma, produced by the fusion of mouse NS1 myeloma cells and splenocytes from a BALB/c mouse. Interleukin-6 (IL6) is mapped on the human chromosome at 7p15.3.

Immunogen

recombinant human IL6

Application

Anti-IL6 high-affinity antibody, mouse monoclonal may be used in:
• immunoblotting
• immunoprecipitation
• enzyme-linked immunosorbent assay (ELISA)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Biochem/physiol Actions

Interleukin-6 (IL6) is a multifunctional cytokine that plays a wide variety of biological roles in numerous systems including the immune, nervous and endocrine systems. It is considered to perform as a primary regulator of acute and chronic inflammatory diseases. IL6 is an excellent target for clinical treatment against autoimmune disorders such as rheumatoid arthritis, juvenile idiopathic arthritis and Castleman′s disease. It is overexpressed in the pathogenesis of myeloma patients. IL6 might have elevated serum IL6 levels in patients with different kinds of malignant disease. IL6 at higher levels are also clinically implemented as biomarkers for acute coronary syndrome (ACS) , unstable angina, cardiovascular disease, heart attack and stroke.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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