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  • SAB4200734 - Anti-IL6 high affinity antibody, Mouse monoclonal

SAB4200734 Sigma-Aldrich

Anti-IL6 high affinity antibody, Mouse monoclonal

clone IL6-2A3, purified from hybridoma cell culture

Synonym: Anti-B-cell stimulatory factor 2 (BSF-2), Anti-CTL differentiation factor (CDF), Anti-Hybridoma growth factor, Anti-Interferon beta-2 (IFN-beta-2), Anti-Interleukin-6

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, IH-IM, Primary Antibodies
clone   IL6-2A3, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunoblotting: 0.125-0.25 μg/mL using recombinant human IL6 expressed in HEK-293T cells
  immunoprecipitation (IP): 10-20 μg/test using concentrated supernatant of human osteosarcoma MG-63 cells treated with IL1β
species reactivity   human
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
isotype   IgG2a
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P05231
Gene Information   human ... IL6(3569)


General description

Interleukin-6 (IL6) is a multifunctional cytokine.


recombinant human IL6


Anti-IL6 high affinity antibody, Mouse monoclonal has been used in
• immunoblotting
• immunoprecipitation

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Biochem/physiol Actions

Interleukin-6 (IL6) plays a wide variety of important biological roles in the immune, nervous and endocrine system. It is an essential component for hybridoma growth and is found in many supplemental cloning media. IL6 regulates acute and chronic inflammatory diseases and has been proven to be an excellent target for clinical treatment against autoimmune disorders such as rheumatoid arthritis, juvenile idiopathic arthritis and Castleman′s disease. IL6 also plays a key role in the pathogenesis of multiple myeloma.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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