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SAB4200736 Sigma-Aldrich

Anti-S100-A10 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-ANX2LG, Anti-CAL1L, Anti-CLP11

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, S1-SD
clone   polyclonal
biological source   rabbit
application(s)   immunoblotting: 1:500-1:1000 using human epidermoid carcinoma A431 cell line extract
  immunohistochemistry: 1:500-1:1000 using heat-retrieved formalin-fixed, paraffin-embedded Human Pancrease carcinoma sections
species reactivity   human
mol wt   ~11 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P60903
Gene Information   human ... S100A10(6281)

Description

General description

S100 calcium binding protein A10 (S100-A10), also known as calpactin I light chain or p11 or cellular ligand of annexin II, is a member of the S100 family of proteins. S100-A10 is mapped to human chromosome 1q21.3 and is expressed in the intestine, lungs and kidney. It contains two EF-hand calcium-binding motifs.

Immunogen

synthetic peptide corresponding to a sequence at the N-terminus of human S100-A10, conjugated to KLH

Application

Anti-S100-A10 antibody produced in rabbit may be used in immunoblotting and immunohistochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Biochem/physiol Actions

S100 calcium binding protein A10 (S100-A10) functions as the regulatory subunit of Annexin II (ANX2) to generate the active heterotetramer ANX2/p11 (ANX2T). S100-A10 is implicated in prostate cancer and multiple myeloma and enables cells to reach adhesion and growth at the bone marrow microenvironment. Elevated levels of S100-A10 are observed in squamous non-cell lung cancer, anaplastic large cell lymphoma, renal cell carcinoma and pediatric intracranial ependymoma. Experimental evidence implicates S100-A10 involvement in the mechanism of action of antidepressant drugs and electroconvulsive seizures due to its interaction with specific serotonin receptors.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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