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SAB4200737 Sigma-Aldrich

Anti-MART-1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-MART-1, Anti-MART1, Anti-MLANA, Anti-Melanoma antigen recognized by T-cells 1

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, M1-MAR, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunoblotting: 2-4 μg/mL using human melanoma SK-Mel-28 cell line extract
  immunofluorescence: 0.25-0.5 μg/mL using human melanoma SK-Mel-28 cell line
  immunohistochemistry: 5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human melanoma sections
species reactivity   human
mol wt   ~13 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q16655
Gene Information   human ... MART-1(2315)

Description

General description

Anti-MART-1 is produced in rabbit using a synthetic peptide corresponding to a sequence from the internal region of human MART-1 protein, conjugated to keyhole limpet hemocyanin (KLH). Melanoma antigen recognized by T-cells 1 (MART-1), also known as melan-A antigen, LB39-AA or antigen SK29-AA, is a type III signal-anchor protein that is localized mainly to the endoplasmic reticulum, the trans-Golgi network and melanosomes. MART-1 protein is expressed specifically in normal skin, retina, melanocyte cell lines and melanomas. It is a melanoma antigen recognized by T-cells and belongs to the melanocyte differentiation markers.

Immunogen

synthetic peptide corresponding to a sequence from the internal region of human MART-1 protein, conjugated to KLH

Application

Anti-MART-1 antibody produced in rabbit has been used in:
• immunoblotting
• immunofluorescence
• immunohistochemistry

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Biochem/physiol Actions

Melanoma antigen recognized by T-cells 1 (MART-1) is specifically recognized by human leukocyte antigen (HLA-A2) restricted tumor-infiltrating T lymphocytes (TIL-1) in melanoma patients and is suggested to be involved in tumor progression. It is also involved in pigmentation process and is suggested as a candidate marker for albinism.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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