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  • SAB4200739 - Anti-Protein kinase C antibody, Mouse monoclonal

SAB4200739 Sigma

Anti-Protein kinase C antibody, Mouse monoclonal

clone MC5, purified from hybridoma cell culture

Synonym: Anti-PKC



Related Categories Alphabetical Index, Antibodies, PR-PR, Primary Antibodies
biological source   mouse
clone   MC5, monoclonal
form   buffered aqueous solution
mol wt   antigen mol wt ~80 kDa
species reactivity   fish, human, mouse, rat, chicken, bovine, rabbit
concentration   ~1.0 mg/mL
application(s)   immunoblotting: 1.25-2.5 μg/mL using human bone marrow neuroblast SH-SY5Y cell extract
  immunocytochemistry: suitable
  immunofluorescence: suitable
  immunoprecipitation (IP): suitable
isotype   IgG2a
shipped in   dry ice
storage temp.   −20°C


Physical form

Purified bovine brain protein kinase C

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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NONH for all modes of transport


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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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