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  • SAB4200746 - Anti-α-Fetoprotein (AFP) antibody, Mouse monoclonal

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SAB4200746 Sigma-Aldrich

Anti-α-Fetoprotein (AFP) antibody, Mouse monoclonal

clone C3, purified from hybridoma cell culture

Synonym: Anti-Alpha-1-fetoprotein, Anti-Alpha-fetoglobulin, Anti-AFP

  •  NACRES NA.46

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Properties

Related Categories Alphabetical Index, Antibodies, FB-FJ, Primary Antibodies
conjugate   unconjugated
clone   C3, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  dot blot: suitable
  immunoblotting: 2-4 μg/mL using liver hepatocellular carcinoma HepG2 cell line extract
  immunofluorescence: suitable
  immunohistochemistry: 2-4 μg/mL using formalin-fixed, paraffin-embedded human fetal liver sections
species reactivity   porcine, canine, human
mol wt   ~74 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
isotype   IgG2a
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P02771
Gene Information   human ... AFP(174)

Description

General description

The gene encoding α-fetoprotein (AFP) is localized on human chromosome 4. It encodes a plasma glycoprotein that has a molecular mass of 70kDa. It is synthesized along with serum albumin during the development of embryonic yolk sac and the liver.

Immunogen

Purified human α-fetoprotein

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Biochem/physiol Actions

α-fetoprotein (AFP) is involved in the regulation of fatty acids in both fetal and proliferating adult liver cells. Its expression is found to be increased in acute liver injuries, indicating active liver regeneration. AFP has been associated with fatty liver disease (FLD), a disease that may lead to cirrhosis and hepatocellular carcinoma. It serves as a tumor marker for HCC (hepatocellular carcinoma).

Other Notes

In order to obtain best results in different techniques and preparations, we recommend determining optimal working concentration by titration test.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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