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  • SAB4200753 - Anti-phospho-AKT (pThr450) antibody, Mouse monoclonal

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SAB4200753 Sigma-Aldrich

Anti-phospho-AKT (pThr450) antibody, Mouse monoclonal

clone AK-11, purified from hybridoma cell culture

Synonym: Anti-Protein Kinase B, Anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1

  •  NACRES NA.44

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Properties

Related Categories Alphabetical Index, Antibodies, PH-PI, Primary Antibodies
clone   AK-11, monoclonal
biological source   mouse
application(s)   immunoblotting: 1-2 μg/mL using human breast cancer cell line MCF-7 extract
  immunofluorescence: 1-2 μg/mL using mouse embryo fibroblast NIH-3T3 cells
  immunoprecipitation (IP): 2-4 μg/test using whole extract of mouse embryo fibroblast NIH-3T3 cell line
species reactivity   human, chicken, rat, mouse
mol wt   ~56 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
isotype   IgG2a
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P31749
Gene Information   human ... AKT1(207)

Description

General description

Anti-phospho-AKT (pThr450) antibody, mouse monoclonal (mouse IgG2a isotype) is derived from the AK-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Serine/threonine kinase (AKT) also known as protein kinase B (PKB) belongs to the family of serine/threonine kinases. It consists of three isoforms such as AKT1, AKT2 and AKT3. AKT1 gene is located on the human chromosome at 14q32.33.

Immunogen

Synthetic peptide corresponding to human AKT1 pThr450

Application

Anti-phospho-AKT (pThr450) antibody, mouse monoclonal may be used in:
• immunoblotting
• immunofluorescence
• immunoprecipitation

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Biochem/physiol Actions

Serine/threonine kinase (AKT) plays an important role in the control of cell cycle and cell proliferation. It is also involved in cell differentiation and apoptosis signaling pathways. AKT is rapidly activated in response to cell stimulation by several growth factors, such as insulin, peroxyvanadate or cellular stresses (such as heat shock). Following activation, the phosphorylated AKT is relocated to the nucleus. Lack of phosphorylation at Thr450 enhances AKT ubiquitination and degradation.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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