• USA Home
  • SAB4200774 - Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal

EMAIL THIS PAGE TO A FRIEND
SAB4200774 Sigma-Aldrich

Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal

clone 13C4, purified from hybridoma cell culture

Synonym: Anti-SLT-1 B subunit, Anti-SLT-1b, Anti-Shiga-like toxin 1 subunit B, Anti-Verocytotoxin 1 subunit B, Anti-Verotoxin 1 subunit B, Anti-stxB

Purchase

Properties

Related Categories Antibodies, Microbiome Antibodies More...
biological source   mouse
antibody product type   primary antibodies
clone   13C4, monoclonal
form   buffered aqueous solution
mol wt   mol wt ~7 kDa
species reactivity   E. coli
concentration   ~1.0 mg/mL
application(s)   flow cytometry: 2.5-5 μg/test using human RAMOS cells pretreated with recombinant Shiga toxin 1, B subunit
  immunoblotting: 1-2 μg/mL using purified recombinant Shiga Toxin 1-B subunit produced in E. coli (using PVDF membrane)
  immunoprecipitation (IP): suitable
isotype   IgG1
UniProt accession no.   Q7BQ98
shipped in   dry ice
storage temp.   −20°C

Description

General description

The Shiga toxins are a family of related protein toxins secreted by certain types of bacteria. Shiga toxin (Stx) is produced by Shigella dysenteriae; whereas, the Shiga-like toxins, Stx1 and Stx2, with a few known isoforms, are secreted by specific strains of Escherichia coli named Shiga-toxin-producing E. coli (STEC) such as E. coli O157:H7. The latter may cause bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications. Monoclonal Anti-Shiga Toxin 1, B Subunit antibody specifically recognizes the B subunit of Shiga holotoxin.

Immunogen

purified Shiga-like toxin from E.coli H30

Application

The product may be used in several immunochemical techniques including immunoblotting (~7 kDa), flow cytometry, and immunoprecipitation. The antibody has been demonstrated to neutralize the cytotoxic activity of the Shiga toxin in cells and the lethality of the toxin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?