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  • SAB4200774 - Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal

SAB4200774 Sigma-Aldrich

Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal

clone 13C4, purified from hybridoma cell culture

Synonym: Anti-SLT-1 B subunit, Anti-SLT-1b, Anti-Shiga-like toxin 1 subunit B, Anti-Verocytotoxin 1 subunit B, Anti-Verotoxin 1 subunit B, Anti-stxB



Related Categories Antibodies, Microbiome Antibodies More...
biological source   mouse
antibody product type   primary antibodies
clone   13C4, monoclonal
form   buffered aqueous solution
mol wt   mol wt ~7 kDa
species reactivity   E. coli
concentration   ~1.0 mg/mL
application(s)   flow cytometry: 2.5-5 μg/test using human RAMOS cells pretreated with recombinant Shiga toxin 1, B subunit
  immunoblotting: 1-2 μg/mL using purified recombinant Shiga Toxin 1-B subunit produced in E. coli (using PVDF membrane)
  immunoprecipitation (IP): suitable
isotype   IgG1
UniProt accession no.   Q7BQ98
shipped in   dry ice
storage temp.   −20°C


General description

The Shiga toxins are a family of related protein toxins secreted by certain types of bacteria. Shiga toxin (Stx) is produced by Shigella dysenteriae; whereas, the Shiga-like toxins, Stx1 and Stx2, with a few known isoforms, are secreted by specific strains of Escherichia coli named Shiga-toxin-producing E. coli (STEC) such as E. coli O157:H7. The latter may cause bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications. Monoclonal Anti-Shiga Toxin 1, B Subunit antibody specifically recognizes the B subunit of Shiga holotoxin.


purified Shiga-like toxin from E.coli H30


The product may be used in several immunochemical techniques including immunoblotting (~7 kDa), flow cytometry, and immunoprecipitation. The antibody has been demonstrated to neutralize the cytotoxic activity of the Shiga toxin in cells and the lethality of the toxin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Safety & Documentation

Safety Information

NONH for all modes of transport
Protocols & Articles


Antibody Basics

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