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SAB4200778 Sigma-Aldrich

Anti-NRAS (internal) antibody produced in rabbit

affinity isolated antibody

Synonym: Anti-GTPase NRas, Anti-transforming protein N-Ras

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Properties

species reactivity   human
application(s)   immunoblotting: 2.5-5 μg/mL using human MCF7 cell line extract
  immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human melanoma sections
clone   polyclonal
concentration   ~1.0 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   predicted mol wt ~21 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... NRAS(4893)
biological source   rabbit
antibody product type   primary antibodies
UniProt accession no.   P01111

Description

Immunogen

Synthetic peptide from the internal region of human NRAS protein, conjugated to KLH

General description

NRAS, also known as GTPase NRas, Transforming protein N-Ras, or Neuroblastoma RAS Viral (V-Ras) Oncogene Homolog, is a member of the Ras protein family, together with KRAS (Kirsten RAS), and HRAS (Harvey RAS), comprising a family of low-molecular-weight GTPases. The Ras family is named for a retrovirus that induced rat sarcomas that were later found to have activating RAS mutations. Ras family proteins serve as molecular switches in regulating pathways that are responsible for diverse cellular processes such as proliferation, differentiation, migration, and apoptosis.3 Ras proteins are highly homologous regarding their primary amino acid sequence and the differences among them concentrated in their C-terminal region. Anti-NRAS (internal) antibody produced in rabbit specifically recognizes human NRAS and does not cross-react with KRAS.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative.

Application

The antibody may be used in various immunochemical techniques including immunoblotting (predicted ∼21 kDa) and immunohistochemistry. Detection of the NRAS band by Immunoblotting is specifically inhibited by the immunizing protein.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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