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SAB5600038 Sigma-Aldrich

Anti-Gamma H2AX (phospho-Ser139) antibody, Rabbit monoclonal

recombinant, expressed in HEK 293 cells, clone RM224, purified immunoglobulin

Synonym: H2AX Ser139 p, Histone H2AX (phospho ser139)

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Properties

Related Categories Alphabetical Index, Antibodies, G-GC, Primary Antibodies, Recombinant Rabbit Monoclonal Antibodies More...
clone   RM224, monoclonal
  recombinant monoclonal
application(s)   ELISA: 0.2- 1  μg/mL
  immunoblotting: 0.5-2 μg/mL
  immunocytochemistry: 0.5-2 μg/mL
species reactivity   human
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG
antibody product type   primary antibodies
recombinant   expressed in HEK 293 cells
concentration   ~1 mg/mL
NCBI accession no.   3014

Description

General description

Histone H2AX (H2A histone family member X), a histone variant present in almost all eukaryotes, is coded by H2AFX gene. It is mapped to human chromosome 11q23.2–11q23.3.

Specificity

This antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.

Immunogen

Synthetic peptide corresponding to Phospho-Histone H2AX (Ser139)

Physical form

Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

H2AX (H2A histone family member X) helps to maintain genome stability. It plays an important role in localisation and structuring of the repair focus. H2AX also plays an active role in supplying the active repair proteins. γH2AX participates in DSB repair. This protein serves as a signal and target of phosphorylation in the spreading phase.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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