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  • SAB5600047 - Anti-BRAF (V600E) antibody, Rabbit monoclonal

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SAB5600047 Sigma-Aldrich

Anti-BRAF (V600E) antibody, Rabbit monoclonal

recombinant, expressed in HEK 293 cells, clone RM8, purified immunoglobulin

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Properties

Related Categories Alphabetical Index, Antibodies, BL-BZ, Primary Antibodies, Recombinant Rabbit Monoclonal Antibodies More...
clone   RM8, monoclonal
  recombinant monoclonal
application(s)   ELISA: 0.5-2 μg/mL
  immunoblotting: 0.5-2 μg/mL
  immunocytochemistry: 0.5-5 μg/mL
  immunohistochemistry: 0.5-5 μg/mL
species reactivity   human
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG
antibody product type   primary antibodies
recombinant   expressed in HEK 293 cells
concentration   ~1 mg/mL
NCBI accession no.   673
UniProt accession no.   P15056

Description

General description

BRAF (B-Raf proto-oncogene) is a serine-threonine kinase, that codes for B-Raf protein. It is located on human chromosome 7q34.

Specificity

This antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.

Immunogen

Peptide corresponding to BRAF V600E mutant

Physical form

Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

BRAF (B-Raf proto-oncogene) participates in signaling direct cell growth. It induces tumor growth by initiating the mitogen-activated protein kinase (MAPK) pathway in various tumors like cutaneous melanoma and carcinomas of the thyroid and colon.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Recombinant Rabbit Monoclonal Antibodies for Immunohistochemistry Applications

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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