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SAE0005 Sigma-Aldrich

Glucose-6-phosphate Isomerase human

recombinant, expressed in E. coli

Synonym: GPI, PGI

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Properties

Quality Level   200
recombinant   expressed in E. coli
assay   ≥95% (SDS-PAGE)
activity   ≥200 units/mg protein
storage temp.   −20°C

Description

Biochem/physiol Actions

Glucose-6-phosphate isomerase (GPI) is a homodimeric enzyme ubiquitously present in most organisms. GPI catalyzes the interconversion between glucose-6-phosphate and fructose-6-hosphate, the second step of the glycolytic pathway. In mammals, Glucose-6-phosphate isomerase also acts as an autocrine motility factor (AMF), a neuroleukin, and a maturation factor. GPI deficiency is the second most common erythroenzymopathy of glycolytic enzymes after pyruvate kinase deficiency. Inherited deficiency of the enzymatic activity of GPI causes hereditary nonspherocytic hemolytic anemia (HNSHA) in humans, a severe deficiency that can be associated with hydrops fetalis (a-thalassaemia), immediate neonatal death, and neurological impairment.

Physical properties

C-terminal histidine-tagged

Unit Definition

One unit will convert 1.0 mmole of D-fructose 6-phosphate to D-glucose 6-phosphate per inute at pH 7.4 at 25 °C.

Physical form

Supplied as a solution in 50 mM Tris-HCl pH 7.5, and 50% glycerol

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
Peer-Reviewed Papers
15

References

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