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SAE0044 Sigma-Aldrich

Choline Oxidase from Arthrobacter sp.

recombinant, expressed in E. coli

Synonym: Choline: oxygen 1-oxidoreductase

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Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates More...
Quality Level   200
recombinant   expressed in E. coli
activity   ≥12 units/mg protein
storage temp.   −20°C

Description

General description

The codA gene encodes the choline oxidase enzyme.

Application

Choline oxidase from Sigma has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection.†

Biochem/physiol Actions

Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the GMC-oxidoreductase family. The enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde, in two sequential FAD-dependent reaction steps. The enzyme is useful for enzymatic determination of phosphatidylcholine by coupling with phospholipase D<REFERENCE ID="34365" TYPE="literature"/> or determination of sphingomyelin by coupling with sphingomyelinase<REFERENCE ID="611299" TYPE="literature"/> and for cholinesterase activity assays.<REFERENCE ID="611300" TYPE="literature"/>
Choline oxidase, isolated from the soil bacterium Arthrobacter globiformis helps to transform choline to glycinebetaine (N-trimethylglycine).

Unit Definition

One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline.

Preparation Note

It is recommended to reconstitute choline oxidase in phosphate buffer at pH 7.5 supplemented with 10mM EDTA. Reconstituted material can be stored at 2-8 °C for at least 2 weeks.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

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Peer-Reviewed Papers
15

References

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