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SAE0045 Sigma-Aldrich

HRV-3C Protease.

N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

Synonym: Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease

  •  NACRES NA.54

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Proteases & Protein Sequencing, Proteases for Recombinant Protein Fusion Tag Cleavage More...
Quality Level   200
assay   ≥90% (SDS-PAGE)
form   aqueous solution
concentration   0.8-1.2 mg/mL
shipped in   dry ice
storage temp.   −20°C

Description

General description

HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.

Application

HRV-3C Protease has been used to remove the GST tag from the DsCystatin protein.

Unit Definition

One unit of HRV3C is defined as amount of enzyme needed to digest 1nM of the substrate peptide per hour (H-Glu-Ala-Leu-Phe-Gln-pNA) at 0 °C in the reaction buffer consisting of 25mM HEPES pH7.5, 150mM NaCl, 1mM EDTA, 1mM DTT.

Physical form

Supplied in a solution containing 50mM Tris HCl pH 7.5, 0.15 M NaCl, 1mM TCEP and 50% (V/V) glycerol.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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