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SAE0069 Sigma-Aldrich

Cholera Toxin B subunit

recombinant, expressed in HEK 293 cells, subunit A free

Synonym: CTB, CTxB, Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Cholera enterotoxin subunit B, Choleragenoid, ToxB

  •  NACRES NA.32

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, G Protein Function, G Proteins and Cyclic Nucleotides, Reagents More...
Quality Level   200
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
quality   (The protein is recombinant, and therefore has no contamination of the highly toxic Cholera toxin A subunit.)
activity   (Binds to GM1 ganglioside. The binding EC50 is lower than 1 ug/ml)
mol wt   pentamer observed mol wt ~70 kDa by SDS-PAGE (non-reducing) (Under non-reducing conditions, it migrates as pentamer of ~70 kDa, consistent with the pentamerization of CTXB.)
  calculated mol wt 13 kDa
  observed mol wt ~13 & 20 kDa by SDS-PAGE (reducing)
solubility   H2O: soluble 11 mg/mL
UniProt accession no.   P01556
storage temp.   −20°C

Description

General description

The advantage of this product over the native CTXB is that it has no traces of Vibrio cholera, and no contamination of the highly toxic Cholera toxin A subunit. This product is expressed in human HEK 293 cells as a C-terminally his-tagged protein with a calculated molecular mass of 13 kDa (amino acids Thr22-Asn124).

Application

Cholera Toxin B subunit has been used:
• for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
• in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Reconstitution

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 1X PBS

Analysis Note

Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with ≤1 μg of Cholera toxin B subunit per mL.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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