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SHC003V Sigma-Aldrich

MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Transduction Particles

Green fluorescent protein marker to monitor transduction efficiency

Synonym: MISSION® TurboGFP® Control Transduction Particles

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Properties

Related Categories Cell Biology, Functional Genomics and RNAi, General Stem Cell Biology, MISSION Controls in Lentiviral Format, MISSION shRNA,
product line   MISSION®
application(s)   capture ELISA: 106 TU/mL using p24
shipped in   dry ice
storage temp.   −70°C

Description

General description

When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.

TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from the copepoda Pontellina plumata. The TurboGFP transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-CMV-TurboGFP (SHC003). It is a positive control to monitor transduction efficiency.

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

Application

MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Transduction Particles has been used to transduce HCT116 (human colon carcinoma) cell line for 2D culture and to form 3D spheroids. It has also been used to generate fluorescent cell lines by transduction.

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

TurboBeads is a trademark of TurboBeads LLC

TurboGFP is a trademark of Evrogen Co.

Safety & Documentation

Safety Information

RIDADR 
UN 3245 9
WGK Germany 
3

Documents

Certificate of Analysis


Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Enhancing Lentiviral Transduction Efficiency

An experiment to directly compare three methods of lentiviral transduction of Jurkat cells was conducted in order to determine the method that yields the greatest transduction efficiency. Spinoculati...
Andrea Spencer
LSI Edition 25
Keywords: Apoptosis, Centrifugation, Diseases, Gene expression, Methods, Protocols, Transduction, Type, Vectors

Protocols

Lentiviral Transduction Protocol

Prepare mammalian cells so that they are growing exponentially and are no more than 70-80% confluent before transduction.
Keywords: Cell culture, Gene expression, Molecular biology, Transduction

Peer-Reviewed Papers
15

References

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SHC002 MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes
SHC001V MISSION® pLKO.1-puro Empty Vector Control Transduction Particles, Contains no shRNA insert
SHC003 MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency
SHC002V MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, Targets no known mammalian genes
SHC004 MISSION® pLKO.1-puro TurboGFP shRNA Control Plasmid DNA, shRNA sequence targeting tGFP
SHC004V MISSION® pLKO.1-puro TurboGFP shRNA Control Transduction Particles, shRNA sequence targeting tGFP
SHM01 MISSION® ExpressMag® Super Magnetic Kit, Increases transduction efficiency Enhance your lentiviral transduction!
SHM02 MISSION® ExpressMag® 96-Well Magnetic Kit, Increases transduction efficiency Enhance your lentiviral transduction in 96-well plate format!
SHC012V MISSION® pLKO.1-puro-CMV-TagRFP Positive Control Transduction Particles, Contains a gene encoding TagRFP
SHC014V MISSION® pLKO.1-puro-UbC-TurboGFP Positive Control Transduction Particles, Contains a gene encoding TurboGFP, under the control of the UbC promoter

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