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SML1734 Sigma-Aldrich

FHZ

≥98% (HPLC)

Synonym: 2-Amino-3′-hydroxy-6′-(2-(2-(2-hydroxyethoxy)ethoxy)ethoxy)spiro[isoindoline-1,9′-xanthen]-3-one

  • CAS Number 1883737-63-0

  • Empirical Formula (Hill Notation) C26H26N2O7

  • Molecular Weight 478.49

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Properties

Related Categories Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules, Cell Biology, Cell Signaling and Neuroscience, Cell Stress,
assay   ≥98% (HPLC)
form   powder
storage condition   protect from light
color   white to light brown
solubility   DMSO: 30 mg/mL, clear
storage temp.   2-8°C

Description

Packaging

25 mg in glass bottle

Biochem/physiol Actions

FHZ is a highly selective, cell-permeable, non-cytotoxic, non-fluorescent 6-triethylene glycol-substituted fluorescein hydrazide that is an efficient fluorescent turn-on probe for dual channel detection of (•OH) and HOCl in live cells and zebrafish embryos. FHZ selectively reacts with hydroxyl (•OH) and HOCl, respectively, to form highly fluorescent FOBA (Ex: 410 nm & Em.: 486 nm; Φ = 0.42; ε410 = 12000/M/cm) and F-TEG (Ex: 490 nm & Em.: 520 nm; Φ = 0.34; ε490 = 7000/M/cm), while exhibiting no reactivity toward H2O2 1O2, O2•–, NO, or ONOO–. Cyan and green fluorescence dual-channel detection with two exciting wavelengths (405 nm and 488 nm) and two collection windows (430–490 nm; 510–560 nm) allows time-dependent quantitative detection of cellular •OH and HOCl by FHZ in live cells (50-100 μM; HeLa and RAW 264.7 macrophages) cells and zebrafish embryos (50 μM).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
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