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SRE0093 Sigma

β-Glucuronidase from limpets (Patella vulgata)

aqueous solution

Synonym: β-D-Glucuronide glucuronosohydrolase

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Properties

Related Categories β-Glucuronidase, β-Glucuronidase Drug Analysis Bundle, Analytical and Industrial Enzymes, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates,
recombinant   expressed in proprietary host
form   aqueous solution
shipped in   ambient
storage temp.   2-8°C

Description

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

More on the suitability of this product for hydrolysis of different drug-glucuronide substrates is available at this page.

Poster presentation comparing the hydrolysis efficiency of novel beta-glucuronidase enzymes from recombinant limpet and recombinant E. coli sources, click at the following poster link: Hydrolysis Efficiency Evaluation of Novel Recombinant Limpet and E. coli Beta-Glucuronidase Enzymes

The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

Features and Benefits

Recombinantly expressed and purified to eliminate MAM esterase activity (6-Monoacetylmorphine→morphine). Representative analysis at high enzyme titer (50 un/μL β-glucuronidase) was less than 1% conversion of 6-MAM to morphine after 4 hours at 60 °C.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

Physical form

Supplied as a solution in 100mM potassium acetate pH 5.2. It is free of detergents, carbohydrates or any other components that may interfere with sample preparation and analysis.

Protocols & Articles

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS. Here we evaluate the hydrolysis efficiency of β-gluc...
Jim Blasberg
Harkewal Singh, Kevin Ray Sigma-Aldrich, St. Louis, MO
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites

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