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SRP6209 Sigma-Aldrich

sCD100/Semaphorin-4D human

recombinant, expressed in E. coli, ≥97% (SDS-PAGE)

Synonym: A8, BB18, GR3, Semaphorin-4D

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Properties

biological source   human
recombinant   expressed in E. coli
assay   ≥97% (SDS-PAGE)
form   lyophilized powder
mol wt   78.9 kDa
packaging   pkg of 10 μg
  pkg of 50 μg
impurities   <1 EU/μg endotoxin (LAL test)
UniProt accession no.   Q92854
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... CD100(10507)

Description

General description

The Semaphorins are a large family of phylogenetically conserved proteins that play a pivotal role in maintaining homeostasis in the immune system. Twenty members of this family have been identified and categorized into eight subclasses based on sequence similarity and distinctive structural features. CD100, also known as Sema4D, is a 150 kDa transmembrane class IV semaphorin. Studies have shown that CD100 can induce monocyte migration, T-cell activation, and B-cell survival, as well as T/B cell and T/DC “cooperation”. The CD100 precursor contains 862 amino acids, including a 21 aa. signal sequence, a 713 aa. extracellular domain, a 21 aa transmembrane sequence, and a 107 aa cytoplasmic region. The extracellular sequence contains several structural features, including a 479 aa “sema” domain, a 79 aa. Ig-like sequence, and a 52 aa “Plexin-type repeat”. Recombinant soluble CD100 is a 78.9 kDa protein comprising the extracellular domain of CD100 (711 amino acids). SDS-PAGE analysis run under non-reducing conditions shows a mixture of disulfide linked dimer and monomer.

Physical form

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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