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SRP6215 Sigma-Aldrich

Enterokinase human

recombinant, expressed in CHO cells, ≥90% (SDS-PAGE)

Synonym: Enteropeptidase, Serine protease 7, transmembrane protease serine 15

  •  NACRES NA.32

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Properties

biological source   human
recombinant   expressed in CHO cells
assay   ≥90% (SDS-PAGE)
form   lyophilized powder
mol wt   97.5 kDa
packaging   pkg of 10 and 50 μg
impurities   <2 EU/μg endotoxin (LAL test)
UniProt accession no.   P98073
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... ENTK(5651)

Description

General description

The enzyme enterokinase is a serine protease that is encoded by a 3696 nucleotide cDNA that contains an open reading frame of 3057 nucleotides. The encoded precursor is cleaved to form an active protein that contains a 784 amino acid heavy chain followed by a 235 amino acid light chain that are linked by one or more disulfide bonds. The mRNA is found to be expressed only in small intestine and the protein is found in enterocytes of duodenum and proximal jejunum . The human enterokinase gene is localized to chromosome 21q21.

Application

Enterokinase has been used in the activation of pancreatic enzyme preparation. It has been used for the activation and determination of trypsin activity in pancreatic enzyme secretion.

Biochem/physiol Actions

The enterokinase enzyme is a glycoprotein that catalyzes the conversion of trypsinogen to trypsin, which activates the zymogens pancreatic digestive enzymes. Deficiency of this enzyme causes protein deficiency as zymogens do not get activated. Congenital deficiency of enteropeptidase leads to severe intestinal malabsorption with diarrhea, vomiting, and growth failure.

Physical form

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5 and 1 mM Calcium Chloride.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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