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SRP6216 Sigma-Aldrich

sFas Ligand human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

Synonym: APTL, Apo I Ligand, CD95L, TNFSF6, soluble Fas Ligand (sFasL)

  •  NACRES NA.32

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Properties

biological source   human
recombinant   expressed in E. coli
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
mol wt   17.9 kDa
packaging   pkg of 10 μg
  pkg of 50 μg
impurities   <1 EU/μg endotoxin (LAL test)
UniProt accession no.   P25445
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... FASL(355)

Description

General description

Fas Ligand (FasL) is a member of the TNF superfamily that is expressed on the cell surface of activated T cells. Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of procaspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. Both human and murine sFasL are fully active on human and murine cells. Recombinant human soluble Fas Ligand is a 17.9 kDa protein comprising the TNF homologous region of FasL and contains an 8 residue N-terminal His-Tag.

Physical form

Sterile filtered through a 0.2 micron filter. Lyophilized from 0.5x PBS, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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