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SRP6226 Sigma-Aldrich

Nesfatin-1 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

Synonym: Nesfatin 1

  •  NACRES NA.32

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Properties

biological source   human
recombinant   expressed in E. coli
assay   ≥98% (SDS-PAGE)
form   lyophilized powder
mol wt   9.7 kDa
packaging   pkg of 10 and 50 μg
impurities   <1 EU/μg endotoxin (LAL test)
UniProt accession no.   P80303
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... NEFA(4925)

Description

General description

Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the precursor protein, Nucleobindin2 (NUCB2). Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets β-cells, gastric endocrine cells and adipocytes. Nesfatin-1 suppresses food intake and can regulate energy metabolism in a Leptin independent manner. Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues.

Physical form

Sterile filtered through a 0.2 micron filter. Lyophilized from 0.1% TFA.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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