SRP6233 Sigma-Aldrich

Relaxin-3 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

Synonym: H3 relaxin, INSL7, Insulin-like peptide-7

  •  NACRES NA.32



biological source   human
recombinant   expressed in E. coli
assay   ≥98% (SDS-PAGE)
form   lyophilized powder
mol wt   5.5 kDa
packaging   pkg of 10 μg
  pkg of 50 μg
impurities   <1 EU/μg endotoxin (LAL test)
UniProt accession no.   Q8WXF3
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... RLN3(117579)


General description

RLN3 (relaxin-3) is a relaxin peptide, belonging to the insulin superfamily, with each member containing two peptide subunits linked by three disulfide bridges. This protein shows a restricted expression pattern in the brain, mainly in lateral periaqueductal gray, nucleus incertus, and central gray regions in the brainstem. This protein is highly conserved across a range of species from fish to mammals.

Biochem/physiol Actions

RLN3 (relaxin-3) functions as a ligand for the leucine-rich repeat-containing G-protein-coupled receptor (LGR), LGR7. It also acts as a ligand for orphan receptor GPCR135, or SALPR (somatostatin- and angiotensin-like peptide receptor). RLN3 acts on LGR7 resulting in stimulation of adenylate cycle; RLN3 acts on GPCR135 to prevent activation of adenylate cyclase. The RLN3–GPCR135 signaling pathway, functions alongside GABA neurotransmitter in specific brain areas, and has regulatory effects on putative GABA neuronal networks. It also interacts with pathways containing CRF (corticotrophin releasing hormone)-like peptides and their receptors. CRF-like peptides and their receptors are implicated in stress-induced autonomic and behavioral changes. RLN3–GPCR135 system is overall involved in arousal, learning and memory, stress, and locomotor activity.

Physical form

Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.


Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Request a Serum Sample
Protocols & Articles
Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?