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SRP6253 Sigma-Aldrich

EGF human

recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE)

Synonym: EGF, HOMG4, URG, epidermal growth factor

  •  NACRES NA.32

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Properties

Related Categories Cell Culture, Epidermal Growth Factors (EGF), Growth Factors and Cytokines, Reagents and Supplements
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥97% (SDS-PAGE)
form   lyophilized powder
mol wt   calculated mol wt 6.3 kDa
  observed mol wt 6.3 kDa by SDS-PAGE (DTT-reduced.)
packaging   pkg of 10 μg
impurities   <1 EU/μg endotoxin (LAL test)
UniProt accession no.   P01133
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... EGF(1950)

Description

General description

The gene EGF (epidermal growth factor) is mapped to human chromosome 4q25-27. The gene encodes for a large type I membrane–anchored precursor protein which is cleaved into pro-EGF for generation of peptide hormone EGF. The mRNA is expressed in the kidney, salivary gland, cerebrum and prostate. Recombinant human EGF is a 6.2kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds.

Application

EGF human has been used to check the action of EGF on lung epithelial NOS (nitric oxide synthase) in A549 cells (adenocarcinoma human alveolar basal epithelial cells). It has been used in the tumor sphere medium for culturing of brain tumor cells.

Biochem/physiol Actions

EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. It signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). Mutation in the EGF gene might be associated with risk of non-small cell lung adenocarcinoma. It is also involved in anaplastic thyroid cancer and renal hypomagnesemia. EGF controls cell differentiation and neurotrophic processes.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

EGF/EGFR signaling pathway

Background Epidermal Growth Factor Receptors (EGFR) and ligands     Receptor activation EGFR Signaling     Ras/Raf signaling cascade     Phosphatidylinositol 3-kinase/Akt signaling cascade     Signal...
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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
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Peer-Reviewed Papers
15

References

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