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SRP6269 Sigma-Aldrich

MMP-1 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym: CLG, CLGN

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Signaling Enzymes, Enzymes, Inhibitors, and Substrates, MMP Enzymes,
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
mol wt   calculated mol wt 52.7 kDa
  observed mol wt 52-55 kDa (DTT-reduced. Phe 20 is the predicted N-terminus.)
packaging   pkg of 10 and 50 μg
impurities   <1 EU/μg endotoxin (LAL test)
conjugate   6-His tagged (N-terminus)
UniProt accession no.   P03956
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MMP-1(4312)

Description

General description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive pro-proteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate the effective levels of matrix metalloproteinases (MMPs) 2 and 9, which are key effectors of ECM remodeling. Matrix metalloproteinase-1 (MMP-1) also known as interstitial collagenase and fibroblast collagenase. MMP1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP-1 breaks down the interstitial collagens, types I, II, and III. MMP1 can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, myelin basic protein, L Selectin, proTNF, IL1β, IGFBP3, IGFBP5, pro MMP2 and pro MMP9.

Physical form

Lyophilized from 0.22 μm filtered solution in MES, NaCl, CaCl2 and Brij­35. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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