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SRP6272 Sigma-Aldrich

SPAM1 human

recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)

Synonym: HYA1, HYAL1, HYAL3, HYAL5, PH-20, SPAG15

  •  NACRES NA.32

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Properties

biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥92% (SDS-PAGE)
form   lyophilized powder
mol wt   calculated mol wt 52.1 kDa
  observed mol wt 64-66 kDa (DTT-reduced. Protein migrates due to different glycosylation. Leu 36 is the predicted N-terminus.)
packaging   pkg of 10 μg
  pkg of 50 μg
impurities   <1 EU/μg endotoxin (LAL test)
conjugate   6-His tagged (C-terminus)
UniProt accession no.   P38567
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... SPAM1(6677)

Description

General description

Hyaluronidase PH-20 also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N – acetyl – β – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Physical form

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.0-7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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